ID-Vit® Vitamin B6 Direct MTP Assay

The ID-Vit® Vitamin B6 Direct MTP Assay is a microtiter plate test kit based on a microbiological assay that measures the vitamin B6 content in serum.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

Manufactured by Immundiagnostik AG.

Also available:

• ID-Vit® Vitamin B1 Direct MTP Assay: KIFR001
• ID-Vit® Vitamin B2 Direct MTP Assay: KIFR002S
• ID-Vit® Vitamin B12 Direct MTP Assay: KIFR012

Test Principle of the ID-Vit® Vitamin B6 Direct MTP Assay

The serum samples are enzymatically pre-treated and then transferred into the wells of a microtiter plate coated with Saccharomyces cerevisiae. The addition of vitamin B6 in either standards or samples gives a vitamin B6-dependent growth response until vitamin B6 is consumed.

After incubation at 30 °C for 48 h, the growth of Saccharomyces cerevisiae is measured turbidimetrically at 610–630 nm (alternatively at 540–550 nm) in an ELISA reader and compared to a standard curve generated from the dilution series. The amount of vitamin B6 is directly proportional to the turbidity.