ID-Vit® Folic Acid Direct MTP Assay

The ID-Vit® Folic Acid Direct MTP Assay is a microtiter plate test kit based on a microbiological assay that measures the folic acid content in serum or plasma.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

This is the RUO version of Immundiagnostik part number KIF005.

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Test Principle of the ID-Vit® Folic Acid Direct MTP Assay

The serum samples are pre-treated and diluted with a buffer mixture and then transferred into the wells of a microtiter plate coated with Lactobacillus rhamnosus. The addition of folic acid in either standards or samples gives a folic acid-dependent growth response until folic acid is consumed.

After incubation at 37 °C for 48 hours, the growth of Lactobacillus rhamnosus is measured turbidimetrically at 610–630 nm (alternatively at 540–550 nm) in an ELISA reader and compared to a standard curve generated from the dilution series. The amount of folic acid is directly proportional to the turbidity.