ox-LDL/MDA Adduct ELISA

The ox-LDL/MDA Adduct ELISA is an enzyme immunoassay intended for the quantitative determination of oxidized LDL in EDTA plasma, serum, and dried blood spots.

FDA Class 1 Exempt. For In Vitro Diagnostic Use.

For Laboratory Professional Use Only.

Developed by Immundiagnostik AG.

We also offer an Anti-ox-LDL ELISA.

Test Principle of the ox-LDL/MDA Adduct ELISA

The ox-LDL/MDA Adduct ELISA is a sandwich ELISA to directly measure ox-LDL in human EDTA plasma and serum. Standards, controls, and samples containing human ox-LDL are added to microplate wells coated with high-affinity antibodies.

During the first incubation period, the antibodies immobilized on the wall of the microtiter wells capture the antigen in the samples. After washing away the unbound components from samples, a peroxidase-conjugated antibody is added to each microtiter well. Tetramethyl-benzidine (TMB) is used as a substrate for peroxidase. Finally, an acidic stop solution is added to terminate the reaction. The intensity of the yellow color is directly proportional to the ox-LDL concentration of the sample.

A dose-response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated using the values obtained from the standard. Ox-LDL, present in the samples, is determined directly from this curve.