ID-Vit® Vitamin B1 Direct MTP Assay is a microtiter plate test kit based on a microbiological assay that measures the vitamin B1 content in serum.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

This is the RUO version of Immundiagnostik part number KIF001.

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Test Principle of the ID-Vit® Vitamin B1 Direct MTP Assay

The whole blood samples are pre-treated and diluted with a buffer mixture and then transferred into the wells of a microtiter plate coated with Lactobacillus fermentum. The addition of vitamin B1 in either standards or samples gives a vitamin B1-dependent growth response until vitamin B1 is consumed.

After incubation at 37 °C for 48 hours, the growth of Lactobacillus fermentum is measured turbidimetrically at 610–630 nm (alternatively at 540–550 nm) in an ELISA reader and compared to a standard curve generated from the dilution series. The amount of vitamin B1 is directly proportional to the turbidity.