Test Principle of the ID-Vit® Biotin Direct MTP Assay
The serum samples are diluted and then added into the wells of a microtiter plate coated with Lactobacillus plantarum. The addition of biotin in either standards or samples gives a biotin-dependent growth response until biotin is consumed.
After incubation at 37 °C for 48 hours, the growth of Lactobacillus plantarum is measured turbidimetrically at 610–630 nm (alternatively at 540–550 nm) in an ELISA reader and compared to a standard curve generated from the dilution series. The amount of biotin is directly proportional to the turbidity.