ID-Vit® Biotin Direct MTP Assay

The ID-Vit® Biotin Direct MTP Assay is a microtiter plate test kit based on a microbiological assay that measures the Biotin content in serum.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

Manufactured by Immundiagnostik AG.

Also available:

• Biotin ELISA: KR8141
• Biotin ELISA (Health Canada): K8141

Test Principle of the ID-Vit® Biotin Direct MTP Assay

The serum samples are diluted and then added into the wells of a microtiter plate coated with Lactobacillus plantarum. The addition of biotin in either standards or samples gives a biotin-dependent growth response until biotin is consumed.

After incubation at 37 °C for 48 hours, the growth of Lactobacillus plantarum is measured turbidimetrically at 610–630 nm (alternatively at 540–550 nm) in an ELISA reader and compared to a standard curve generated from the dilution series. The amount of biotin is directly proportional to the turbidity.