The ID-Vit® Biotin Direct MTP Assay is a microtiter plate test kit based on a microbiological assay that measures the Biotin content in serum.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

This is the RUO version of Immundiagnostik part number KIF007.

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Test Principle of the ID-Vit® Biotin Direct MTP Assay

The serum samples are diluted and then added into the wells of a microtiter plate coated with Lactobacillus plantarum. The addition of biotin in either standards or samples gives a biotin-dependent growth response until biotin is consumed.

After incubation at 37 °C for 48 hours, the growth of Lactobacillus plantarum is measured turbidimetrically at 610–630 nm (alternatively at 540–550 nm) in an ELISA reader and compared to a standard curve generated from the dilution series. The amount of biotin is directly proportional to the turbidity.