The ID-Vit® Vitamin B2 Direct MTP Assay is a microtiter plate test kit based on a microbiological method that measures the vitamin B2 content in serum.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

This is the RUO version of Immundiagnostik part number KIF002S.

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Test Principle of the ID-Vit® Vitamin B2 Direct MTP Assay

The serum samples are pre-treated and diluted with a buffer mixture and then transferred into the wells of a microtiter plate coated with Lactobacillus rhamnosus. The addition of vitamin B2 in either standards or samples gives a vitamin B2-dependent growth response until vitamin B2 is consumed.

After incubation at 37 °C for 48 hours, the growth of Lactobacillus rhamnosus is measured turbidimetrically at 610–630 nm (alternatively at 540–550 nm) in an ELISA reader and compared to a standard curve generated from the dilution series. The amount of vitamin B2 is directly proportional to the turbidity.