ID-Vit® Vitamin B12 Direct MTP Assay

The ID-Vit® Vitamin B12 Direct MTP Assay is a microtiter plate test kit based on a microbiological assay that measures the vitamin B12 content in serum.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

Manufactured by Immundiagnostik AG.

Also available:

• • ID-Vit® Vitamin B1 Direct MTP Assay: KIFR001
  • ID-Vit® Vitamin B2 Direct MTP Assay: KIFR002S
  • ID-Vit® Vitamin B6 Direct MTP Assay: KIFR006

Test Principle of the ID-Vit® Vitamin B12 Direct MTP Assay

The serum samples are pre-treated and diluted with a buffer mixture and then transferred into the wells of a microtiter plate coated with Lactobacillus delbrueckii subsp. lactis. The addition of vitamin B12 in either standards or samples gives a vitamin B12-dependent growth response until vitamin B12 is consumed.

After incubation at 37 °C for 48 hours, the growth of Lactobacillus delbrueckii subsp. lactis is measured turbidimetrically at 610–630 nm (alternatively at 540–550 nm) in an ELISA reader, and a standard curve is generated from the dilution series. The amount of vitamin B12 is directly proportional to the turbidity.