ID-Vit® Pantothenic Acid Direct MTP Assay

The ID-Vit® Pantothenic Acid Direct MTP Assay is a microtiter plate test kit based on a microbiological assay for the determination of total pantothenic acid (Vitamin B5 ) in serum using a Lactobacillus Plantarum coated microtitre plate.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

This is the RUO version of Immundiagnostik part number KIF004.

Browse our full offering of ID-Vit® Vitamin Assays.

Test Principle of the ID-Vit® Pantothenic Acid Direct MTP Assay

The serum samples are diluted and then transferred into the wells of a microtiter plate coated with Lactobacillus plantarum. The addition of pantothenic acid in either standards or samples gives a pantothenic acid-dependent growth response until pantothenic acid is consumed.

After incubation at 37 °C for 24 hours, the growth of Lactobacillus plantarum is measured turbidimetrically at 610–630 nm (alternatively at 540–550 nm) in an ELISA reader and compared to a standard curve generated from the dilution series. The amount of pantothenic acid is directly proportional to the turbidity.