ID-Vit® Niacin Direct MTP Assay

The ID-Vit® Niacin Direct MTP Assay is a microtiter plate test kit based on a microbiological assay that measures the total niacin content (nicotinic acid and nicotinamide) in serum, citrate, or EDTA plasma.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

Manufactured by Immundiagnostik AG.

Test Principle of the ID-Vit® Niacin Direct MTP Assay

The serum samples are diluted and then transferred into the wells of a microtiter plate coated with Lactobacillus plantarum. The addition of niacin in either standards or samples gives a niacin-dependent growth response until niacin is consumed.

After incubation at 37 °C for 48 hours, the growth of Lactobacillus plantarum is measured turbidimetrically at 610–630 nm (alternatively at 540–550 nm) in an ELISA reader and compared to a standard curve generated from the dilution series. The amount of niacin is directly proportional to the turbidity.