Lysozyme ELISA (Stool)

The Lysozyme ELISA (Stool) is an enzyme immunoassay intended for the quantitative determination of lysozyme in stool.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

The Lysozyme ELISA (Stool) is compatible with our IDK Extract® Stool Sample Preparation System.

If your lab is testing at high volumes, this kit is also available in a customized bulk pack (20 plates).

This is the RUO version of Immundiagnostik part number K 6900.

Test Principle of the Lysozyme ELISA

The assay utilizes the “sandwich” technique with two selected antibodies that recognize human lysozyme. Standards, controls, and diluted samples, which are assayed for human lysozyme, are added to the wells of a microplate coated with a high affine anti-human lysozyme antibody. During the first incubation step, lysozyme is bound by the immobilized antibody. Then a peroxidase-conjugated anti-human lysozyme antibody is added to each microtiter well, and a “sandwich” of capture antibody – human lysozyme – peroxidase-conjugate is formed. Tetramethylbenzidine is used as peroxidase substrate.

Finally, an acidic stop solution is added to terminate the enzymatic reaction. The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of lysozyme. A dose-response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated using the values obtained from the standards. Lysozyme present in the samples is determined directly from this curve.