Lysozyme ELISA

The Lysozyme ELISA is an enzyme immunoassay intended for the quantitative determination of lysozyme in human serum, urine, and liquor.

For Laboratory Professional Use Only.

Developed by Immundiagnostik AG.

Test Principle of the Lysozyme ELISA

The Lysozyme ELISA utilizes the “sandwich” technique with two selected antibodies that recognize human lysozyme. Standards, controls, and diluted samples, which are assayed for human lysozyme, are added to the wells of a microtiter plate coated with a high affine anti-human lysozyme antibody.

During the first incubation step, lysozyme is bound by the immobilized antibody. Then a peroxidase-conjugated anti-human lysozyme antibody is added into each microtiter well, and a “sandwich” of capture antibody – human lysozyme – peroxidase-conjugate is formed. Tetramethylbenzidine is used as peroxidase substrate.

Finally, an acidic stop solution is added to terminate the enzymatic reaction. The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of lysozyme. A dose-response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated using the values obtained from the standards. The presence of Lysozyme in the samples is determined directly from this curve.