IDK® Calprotectin ELISA

The IDK® Calprotectin ELISA is an enzyme immunoassay intended for the quantitative determination of calprotectin (MRP 8/14, S100A8/A9) in stool.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

If you’re in Canada, we offer a Health Canada Licensed version of this kit: K6927

Manufactured by Immundiagnostik AG.

This kit can be used with the IDK Extract® Stool Collection Tubes: K6999.US

Also available:

• Calprotectin ELISA Bulk Pack (20 Plates): KR6927.20

Test Principle of the IDK® Calprotectin ELISA

The IDK® Calprotectin ELISA is designed for the quantitative determination of Calprotectin. The assay utilizes the two-site sandwich technique with two selected monoclonal antibodies that bind to human Calprotectin. Standards, controls, and diluted samples assayed for human Calprotectin are added to wells of microplate coated with a high affine monoclonal anti-human calprotectin antibody.

During the first incubation step, Calprotectin in the samples is bound by the immobilized antibody. Then, a peroxidase-labeled conjugate is added to each well, and the following complex is formed: capture antibody – human Calprotectin – peroxidase conjugate.

Tetramethylbenzidine (TMB) is used as a substrate for peroxidase. Finally, an acidic stop solution is added to terminate the reaction. The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the calprotectin concentration of the sample.

A dose-response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated using the values obtained from the standard. The presence of Calprotectin in the samples is determined directly from this curve.