Lysozyme ELISA (1-point calibration)

The Lysozyme ELISA (1-point calibration) is an enzyme immunoassay intended for the quantitative determination of Lysozyme in human serum, urine, and liquor.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

This is the RUO version of Immundiagnostik part number K 6903.

Test Principle of the Lysozyme ELISA

The Lysozyme ELISA is designed for the quantitative determination of Lysozyme. The assay utilizes the sandwich technique with two selected antibodies that recognise human Lysozyme. Calibrator, controls, and diluted samples, assayed for human Lysozyme, are added into the wells of a microplate coated with a high affine anti-human lysozyme antibody.

During the first incubation step, Lysozyme is bound by the immobilized antibody. Then a peroxidase-conjugated anti-human lysozyme antibody is added into each microtiter well, and a “sandwich” of capture antibody – human Lysozyme – peroxidase-conjugate is formed. Tetramethylbenzidine is used as a peroxidase substrate.

Finally, an acidic stop solution is added to terminate the enzymatic reaction. The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of Lysozyme. Samples are quantified by referring their optical density to a lot-dependent master calibration curve and using the calibrator run with each test.