The NGAL ELISA is an enzyme-linked immunosorbent assay intended for the quantitative determination of human neutrophil gelatinase-associated lipocalin (NGAL) in urine and EDTA plasma.
For Research Use Only. Not for use in diagnostic procedures.
The NGAL ELISA is a sandwich ELISA performed in microwells coated with a monoclonal antibody to human neutrophil gelatinase-associated lipocalin. Bound NGAL is detected with another monoclonal NGAL antibody labeled with biotin, and the assay develops with horseradish peroxidase (HRP)-conjugated streptavidin followed by the addition of a color-forming substrate.