IDK® MPO ELISA Bulk Pack (Stool, Urine, 20 Plates)
Sample Type (Matrix): Stool, Urine
Sample Volume: 100 µl
Incubation time: 2h 10m
Standard Range: 1.9-30 ng/ml
Size: 20 x 96 wells
Regulatory Status: For research use only in the U.S.
Kit Manual: Download
The IDK® MPO ELISA Bulk Pack (Stool, Urine, 20 Plates) is an enzyme immunoassay intended for the quantitative determination of myeloperoxidase (MPO) in urine and stool.
For research use only. Not for use in diagnostic procedures.
For Laboratory Professional Use Only.
Please inquire about pricing, as each IDK® MPO ELISA Bulk Pack is customized to meet the needs of your lab.
We also offer the IDK® MPO ELISA (Stool, Urine) in a single plate format.
Developed by Immundiagnostik AG.
Test Principle of the IDK® MPO ELISA Bulk Pack
The IDK® MPO ELISA is designed for the quantitative determination of myeloperoxidase (MPO) in urine and stool.
In the first incubation step, the myeloperoxidase in the samples is bound to an available excess of antibodies against myeloperoxidase, which are immobilized to the surface of the microtiter plates. To remove all unbound substances, a washing step is carried out. In a second incubation step, a peroxidase-labeled antibody against MPO is added. After another washing step, to remove all unbound substances, the solid phase is incubated with the substrate, tetramethylbenzidine (TMB). An acidic stop solution is then added to stop the reaction. The color converts from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of MPO in the sample.
A dose-response curve of the absorbance unit (optical density, OD) vs. concentration is generated, using results obtained from the calibrators. The presence of MPO in the samples is determined directly from this curve.