IDK® MPO ELISA (Stool, Urine)

The IDK® MPO ELISA (Stool, Urine) is an enzyme immunoassay intended for the quantitative determination of myeloperoxidase in urine and stool.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

If your lab is testing at high volumes, the IDK® MPO ELISA (Stool, Urine) is also available in a customized bulk pack (20 plates).

This is the RUO version of Immundiagnostik part number K 6630.

Test Principle of the IDK® MPO ELISA (Stool, Urine)

The IDK® MPO ELISA is designed for the quantitative determination of myeloperoxidase (MPO) in urine and stool.

In the first incubation step, the myeloperoxidase in the samples is bound to an available excess of antibodies against myeloperoxidase, which are immobilized to the surface of the microtiter plates. To remove all unbound substances, a washing step is carried out. In a second incubation step, a peroxidase-labeled antibody against MPO is added. After another washing step, to remove all unbound substances, the solid phase is incubated with the substrate, tetramethylbenzidine (TMB). An acidic stop solution is then added to stop the reaction. The color converts from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of MPO in the sample.

A dose-response curve of the absorbance unit (optical density, OD) vs. concentration is generated, using results obtained from the calibrators. The presence of MPO in the samples is determined directly from this curve.