The IDK® Calprotectin (MRP8/14) ELISA is an enzyme immunoassay intended for the quantitative determination of calprotectin (MRP 8/14, S100A8/A9) in serum and plasma.

For research use only in the U.S. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

This is the RUO version of Immundiagnostik part number K 6935.

Developed by Immundiagnostik AG.

Test Principle of the IDK® Calprotectin (MRP8/14) ELISA

The IDK® Calprotectin (MRP8/14) ELISA is designed for the quantitative determination of calprotectin (MRP (8/14, S100A8/A9). The assay utilizes the two-site sandwich technique with two selected monoclonal antibodies that bind to human calprotectin. Standards, controls, and diluted samples that are assayed for human calprotectin are added to wells of microplate coated with a high affine monoclonal anti-human calprotectin antibody.

During the first incubation step, calprotectin in the samples is bound by the immobilized antibody. Then a peroxidase-labeled conjugate is added to each well, and the following complex is formed: capture antibody - human calprotectin – peroxidase conjugate.

Tetramethylbenzidine (TMB) is used as a substrate for peroxidase. Finally, an acidic stop solution is added to terminate the reaction. The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the calprotectin concentration of the sample.

A dose-response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated, using the values obtained from the standard. The presence of calprotectin in the samples is determined directly from this curve.