Porphyrins HPLC Kit

SKU KCR2601 Category

$333.00

Available on backorder

Product Specifications

Method: HPLC
Sample Type (Matrix): Urine
Sample Volume: 1 ml
Species: Human
Standard Range: Gradient
Size: 100 Tests
Regulatory Status: For research use only in the U.S.

Description

The Porphyrins HPLC Kit is intended for the quantitative determination of porphyrins in urine.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

This is the RUO version of Immundiagnostik part number KC2601.

We also offer a Zinc-Protoporphyrin / Protoporphyrin HPLC Kit.

Test Principle of the Porphyrins HPLC Kit

The first step in the determination of porphyrins includes a straightforward sample preparation. The pH value of the sample and the calibrator is adjusted below 2.5 by adding 20 μl of hydrochloric acid. After centrifugation, the supernatant is injected into the HPLC system.

The separation via HPLC follows a gradient method, using a reversed-phase column at 30 °C; one run lasts 25 minutes. The quantification is performed with the delivered calibrator. The concentration is calculated by integrating the peak areas or heights.

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