IDKmonitor® Adalimumab Total ADA ELISA (Health Canada)

The IDKmonitor® Adalimumab Total ADA ELISA (Health Canada) is intended for the determination of human antibodies against TNFα blocker adalimumab (e.g. HUMIRA®) in the presence of adalimumab in EDTA plasma and serum.

Health Canada Licensed.

For Laboratory Professional Use Only.

Not for sale in the U.S. Please contact us to order or get a quote.

If you’re in the U.S., we offer an RUO version of this kit: KR9651

Manufactured by Immundiagnostik AG.

Test Principle of the IDKmonitor® Adalimumab Total ADA ELISA (Health Canada)

This ELISA is designed for the quantitative determination of antibodies against TNFα blocker adalimumab (e. g. HUMIRA®).

During sample preparation, the anti-drug antibodies (ADA) are separated from the therapeutic antibody in order to acquire free ADA. By adding the conjugate (peroxidase-labeled therapeutic antibody) and the tracer (biotinylated therapeutic antibody), the unmarked therapeutic antibodies are replaced, and the marked antibodies can form a complex with the ADA. This complex binds via biotin to the streptavidin-coated microtiter plate. It is detected via the peroxidase conjugate with the peroxidase, converting the substrate TMB to a blue product.

The enzymatic reaction is stopped by adding an acidic solution. The samples convert from blue to yellow. The color change should be measured in a photometer at 450 nm. The interpretation is made using the cut-off control.