IDKmonitor® Adalimumab Total ADA ELISA

The IDKmonitor® Adalimumab Total ADA ELISA is intended for the determination of human antibodies against TNFα blocker adalimumab (e.g. HUMIRA®) in the presence of adalimumab in EDTA plasma and serum.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

This is the RUO version of Immundiagnostik part number K 9651.

IDKmonitor® Resources

Download our IDKmonitor® ELISAs brochure to discover more.

Test Principle of the IDKmonitor® Adalimumab Total ADA ELISA

The IDKmonitor® Adalimumab Total ADA ELISA is designed for the quantitative determination of antibodies against TNFα blocker adalimumab (e. g. HUMIRA®).

During sample preparation, the anti-drug antibodies (ADA) are separated from the therapeutic antibody in order to acquire free ADA. By adding the conjugate (peroxidase-labeled therapeutic antibody) and the tracer (biotinylated therapeutic antibody), the unmarked therapeutic antibodies are replaced, and the marked antibodies can form a complex with the ADA. This complex binds via biotin to the streptavidin-coated microtiter plate. It is detected via the peroxidase conjugate with the peroxidase converting the substrate TMB to a blue product.

The enzymatic reaction is stopped by adding an acidic solution. The samples convert from blue to yellow. The color change should be measured in a photometer at 450 nm. The interpretation is made using the cut-off control.