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IDK® Biotin ELISA K 8141

Item #: K 8141 Category:


In Stock

Method: ELISA
Sample Type (Matrix): Serum, Plasma, Urine, Milk
Sample Volume: 50 µl
Species: Human
Incubation time: 1h 10m
Size: 96 wells
Regulatory Status: Health Canada Licensed.
Kit Manual: Download

The IDK® Biotin ELISA is intended for the quantitative determination of biotin (Vitamin H) in serum, plasma, urine, and milk. Health Canada Licensed.

Biotin (vitamin H) is present in bacteria, funghi, plants, and animals. In food, the major part of biotin is covalently bound to protein, leaving only a minor fraction freely available. During digestion, biocytin (biotinyl-lysine) is released from the proteins and can be resorbed as easily as biotin from the intestinal tract. Afterward, biotin is released from biocytin by the enzyme biocytinase in erythrocytes and plasma. It is then available as a prosthetic group for a series of biotin-dependent enzymes.

The daily requirements of biotin are difficult to estimate because a healthy intestinal flora supplies a major part of biotin by endogenous synthesis. The generally recommended daily dose for adults is 100–200 µg. Chronic hemodialysis patients show clear improvements in neuropathological status and glucose metabolism when supplemented with biotin in a milligram range.

Biotin deficiency can be caused by the destruction of the intestinal flora or extreme diets (e.g. frequent consumption of raw eggs). It can lead to dermatitis, hair loss, anorexia, muscular hypotonia, depression, and reproduction problems.

Additional information

Weight35.5 oz
Dimensions6.5 × 10 × 4.5 in
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