IDK® PMN Elastase ELISA

The IDK® PMN Elastase ELISA is an enzyme immunoassay intended for the quantitative determination of PMN elastase in serum, plasma, and seminal plasma.

This is the RUO version of Immundiagnostik part number K 6841.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

Test Principle of the IDK® PMN Elastase ELISA

In the first incubation step, PMN elastase in the sample is bound to polyclonal rabbit anti-PMN elastase antibodies (in excess), which are immobilized on the surface of the microtiter wells. To remove all unbound substances, a washing step is carried out.

In a second incubation step, a monoclonal mouse-anti-PMN elastase antibody (AB) is added. This antibody is able to detect both the free and the complex form with the specific inhibitor (α1-proteinase inhibitor = α1-antitrypsin). The quantification of the bound PMN elastase is carried out by adding an anti-mouse peroxidase-labeled conjugate. Finally, the PMN elastase – antigen – antibody complex is incubated with the peroxidase substrate, tetramethylbenzidine.

An acidic stop solution is then added to terminate the reaction. The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of PMN elastase in the sample.

A dose-response curve of the absorbance unit (optical density, OD) vs. concentration is generated, using the values obtained from the calibrators. PMN elastase, present in the samples, is determined directly from this curve.

The combination of two specific antibodies in the PMN elastase ELISA drastically reduces the possibility of false results and offers a reliable detection system to the user.