IDK® EDN ELISA

The IDK® EDN ELISA is an enzyme immunoassay intended for the quantitative determination of eosinophil-derived neurotoxin (also known as RNASE2 or eosinophil protein x [EPX]) in serum, plasma, urine, and stool.

For Research Use Only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

Manufactured by Immundiagnostik AG.

This kit can be used with the IDK Extract® Stool Collection Tubes: K6999.US

Also available:

• EDN ELISA Bulk Pack (20 plates)

Test Principle of the IDK® EDN ELISA

The IDK® EDN ELISA utilizes the two-site sandwich ELISA technique with two selected antibodies (monoclonal and polyclonal) that bind to human EDN. Assay standards, controls, and prediluted samples containing human EDN are added to wells of microplate coated with a high affine monoclonal anti-human EDN antibody.

After the first incubation, the antibody immobilized on the wall of microtiter wells captures human EDN in the sample. Then a peroxidase-conjugated rabbit polyclonal anti-human EDN antibody is added to each microtiter well, and a sandwich of capture antibody – human EDN – Peroxidase-conjugate is formed. Tetramethylbenzidine is used as a substrate for peroxidase.

Finally, an acidic stop solution is added to terminate the reaction. The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of EDN.

A dose-response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated using the values obtained from the standard. The presence of EDN n the samples is determined directly from this curve.