Immundiagnostik, Inc. offers the ReFiNE® DKK3 ELISA to help support your lab’s kidney research. Dickkopf-related protein 3 (DKK3), a member of the evolutionarily conserved Dickkopf protein family, plays a role in Wnt/β-catenin signaling. DKK3 has been implicated in the pathogenesis of various diseases and is recognized as a key driver of tubulointerstitial injury and fibrosis. It is secreted by ‘stressed’ tubular epithelial cells in the kidney and can be quantified in urine. Recent findings suggest that urinary DKK3 may serve as a potential biomarker for monitoring kidney disease progression and evaluating the effects of interventions1-4.
The ReFiNE® DKK3 ELISA is intended for the quantitative determination of dickkopf-related protein 3 in human urine samples.
If the samples are not assayed immediately, the supernatant of the centrifuged urine samples must be stored at 2 – 8 °C and assayed within 3 days. For repeated measurements, it is recommended to aliquot the samples and to store them at -20 °C.
Repeated freezing and thawing of samples is possible up to 5 times.
The supernatant of the urine samples must be diluted 1:10 before performing the assay.
Sample Type: Urine
Sample Volume: 100 μL
Incubation Time: 1 hr 15 min
Time-to-result: < 2.5
Standard Range: 16.751-4000 pg/mL
Automatable: Yes
The ReFiNE® DKK3 ELISA is for Research Use Only in North America. Not for Use in Diagnostic Procedures. For Laboratory Professional Use Only.
No content on this site, regardless of date, should ever be used as a substitute for direct medical advice from your doctor or other qualified clinician.
