Vitamin C HPLC Kit

The Vitamin C HPLC Kit is intended for the quantitative determination of vitamin C in plasma.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

Manufactured by Immundiagnostik AG.

Also available:

• Vitamin C Colorimetric Microtiter Plate Assay: KR4000

Test Principle of the Vitamin C HPLC Kit

The first step in determining vitamin C is the precipitation of the higher molecular components. After their removal by centrifugation, the supernatant is injected into the HPLC system.

The vitamin C analysis via HPLC follows an isocratic method at 30 °C using a reversed-phase column. One run lasts 12 minutes. A UV detector records the chromatograms. The quantification is performed with the delivered calibrator. The concentration is calculated by integrating the peak areas/heights by the external standard method.