The first step in determining vitamin C is the precipitation of the higher molecular components. After their removal by centrifugation, the supernatant is injected into the HPLC system.
The vitamin C analysis via HPLC follows an isocratic method at 30 °C using a reversed-phase column. One run lasts 12 minutes. A UV detector records the chromatograms. The quantification is performed with the delivered calibrator. The concentration is calculated by integrating the peak areas/heights by the external standard method.