Vitamin B6 HPLC Kit (Pyridoxal-5-Phosphate)

The Vitamin B6 HPLC Kit Pyridoxal-5-Phosphate is intended for the quantitative determination of vitamin B6 in plasma and serum.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

This is the RUO version of Immundiagnostik part number KC2100.

Browse our other Vitamin HPLC Kits.

Test Principle of the Vitamin B6 HPLC Kit Pyridoxal-5-Phosphate

The first step in determining vitamin B6 includes sample preparation with additional derivatization. During the precipitation, higher molecular substances are removed. After centrifugation, the supernatant is used for derivatization (20 min at 60 °C), transforming the vitamin B6 into a fluorescent product. The sample is cooled, centrifuged, and injected into the HPLC system.

The separation via HPLC follows an isocratic method at 30 °C using a reversed-phase column; one run lasts about 10 minutes. The quantification is performed with the delivered plasma calibrator; the concentration is calculated by integrating the peak area/heights.