The Tissue Transglutaminase Antibody IgA CLIA Kit is intended for the quantitative determination of human tTG-IgA levels in serum.
Compatible with the ECL100 Chemiluminescent Immunoassay Analyzer.
For research use only. Not for use in diagnostic procedures.
For lab professional use only.
Manufactured by Epitope Diagnostics, Inc.
Please contact us for a quote.
The Tissue Transglutaminase Antibody IgA CLIA Kit is designed, developed, and produced for the quantitative measurement of human tTG-IgA in serum samples. The assay utilizes a two-site “sandwich” technique with one antigen and one antibody that bind to different epitopes and paratopes of tTG-IgA.
Assay calibrators, controls, or samples are added directly to a reaction vessel containing streptavidin-coated magnetic particles. Simultaneously, an acridinium ester antibody and a biotin antigen are added. The magnetic particles capture the biotin antigen as well as an immuno complex in the form of “magnetic particles – biotin tTG-IgA antigen –tTG-IgA – acridinium ester tTG-IgA antibody”.
The materials bound to the solid phase are held in a magnetic field while unbound materials are washed away. Then, the trigger solution is added to the reaction vessel, and the light generated by the reaction is measured with the ECL100 Chemiluminescent Immunoassay Analyzer. The relative light units (RLU) are proportional to the concentration of tTG-IgA in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve and reported in serum tTG-IgA concentration.
