The Thymulin ELISA is an enzyme immunoassay intended for the quantitative determination of thymulin in serum and thymus preparations.
For research use only. Not for use in diagnostic procedures.
For Laboratory Professional Use Only.
Manufactured by Immundiagnostik AG.
The Thymulin ELISA test principle is based on a competition between the antigen in the sample or standards and biotinylated thymulin as a tracer for the binding sites of anti-thymulin antibodies coated on the microplate wells.
A peroxidase-conjugated steptavidin is used for detection and quantification, and tetramethylbenzidine (TMB) is used as a peroxidase substrate. An acidic stop solution terminates the enzymatic reaction.
Using the values obtained from the standards, a dose-response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated. This curve determines the presence of thymulin in the samples directly.