Thymosin alpha-1 ELISA

The Thymosin alpha-1 ELISA is an enzyme immunoassay intended for the quantitative determination of thymosin α1 (Tα1) in serum and thymus extract.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

Developed by Immundiagnostik AG.

We also offer a Thymosin beta-4 ELISA.

Test Principle of the Thymosin alpha-1 ELISA

The Thymosin alpha-1 ELISA is designed for the quantitative determination of thymosin a1 (Tα1) in serum and thymus extract.

In this test, polyclonal rabbit antibodies directed against synthetic Tα1 are used. The test principle is based on a competition between antigens in the sample or standards and the antigen coated on the wells of the microplate. A peroxidase-conjugated antibody is used for detection and quantification, and tetramethylbenzidine (TMB) is used as a peroxidase substrate. The enzymatic reaction is terminated by an acidic stop solution.

A dose-response curve of absorbance unit (optical density, OD at 450 nm) vs. concentration is generated using the values obtained from the standard. The presence of Tα1 in the samples is determined directly from this curve.