Prealbumin/Transthyretin ELISA

The Prealbumin/Transthyretin ELISA is intended for the quantitative determination of prealbumin (transthyretin) in serum, plasma, urine, and stool.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

Manufactured by Immundiagnostik AG.

Test Principle of the Prealbumin/Transthyretin ELISA

The Prealbumin/Transthyretin ELISA is designed for the quantitative determination of human prealbumin (transthyretin) in serum, plasma, urine, and stool.

Standards, controls, and samples containing human prealbumin (transthyretin) are added to the wells of a microplate coated with polyclonal anti-human prealbumin antibodies. The antibodies immobilized on the walls of the microtiter wells capture prealbumin in the samples during the first incubation step. After washing away the unbound components, a peroxidase-conjugated anti-prealbumin detection antibody is added to each well.

During a second incubation, the detection antibody is bound to the captured prealbumin. A sandwich of capture antibody – human prealbumin – peroxidase conjugate is formed.

Tetramethylbenzidine (TMB) is used as a peroxidase substrate. Finally, an acidic stop solution is added to terminate the reaction. The intensity of the yellow color is directly proportional to the prealbumin concentration of the sample.

A dose-response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated using the values obtained from the standard. The presence of prealbumin (transthyretin) in the samples is determined directly from this curve.