The Pepsinogen II CLIA Kit is a chemiluminescent immunoassay (CLIA) kit intended for the quantitative determination of Pepsinogen II levels in serum.
Compatible with the ECL100 Chemiluminescent Immunoassay Analyzer.
For Research Use Only. Not for use in diagnostic procedures.
For Laboratory Professional Use Only.
Manufactured by Epitope Diagnostics, Inc.
Please contact us for a quote.
This CLIA is designed, developed, and produced for the quantitative measurement of human Pepsinogen II levels in serum samples. The assay utilizes a two-site “sandwich” technique with two antibodies that bind to different epitopes of Pepsinogen II.
Assay calibrators, controls, or samples are added directly to a reaction vessel containing streptavidin-coated magnetic particles. Simultaneously, an acridinium ester antibody and a biotin antibody are added. The magnetic particles capture the biotin antibody and an immunocomplex in the form of “magnetic particles – biotin pepsinogen II antibody – pepsinogen II – acridinium ester pepsinogen II antibody.”
The materials bound to the solid phase are held in a magnetic field while unbound materials are washed away. Then, the trigger solution is added to the reaction vessel, and the light generated by the reaction is measured with the ECL100 Chemiluminescent Immunoassay Analyzer. The relative light units (RLU) are proportional to the concentration of pepsinogen II in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve and reported in serum pepsinogen II concentration.
