Osteoprotegerin (Mouse/Rat) ELISA

The Osteoprotegerin (Mouse/Rat) ELISA is a sandwich ELISA intended for the determination of mouse/rat osteoprotegerin (OPG) in serum, plasma, urine, and cell culture supernatants.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

Developed by Immundiagnostik AG.

Test Principle of the Osteoprotegerin (Mouse/Rat) ELISA

The Osteoprotegerin (Mouse/Rat) ELISA is an assay for the direct determination of OPG in rodent serum, plasma, and urine. Two highly specific antibodies against OPG are used. The capture antibody is attached to the microtiter plate wells, and the detection antibody is labeled with biotin.

In the first incubation step, the samples and the biotinylated antibody against OPG react with the coated capture antibody on the microtiter plate. A sandwich-type complex forms consisting of the binding antibody on the plate, OPG, and the biotinylated detection antibody. A washing step is carried out to remove all unspecific bound substances.

In the second step, streptavidin-peroxidase is added, which reacts with the detection antibody. After another washing step, the solid phase is incubated with the substrate, tetramethylbenzidine. An acidic stopping solution is subsequently added, and the blue color changes to yellow. The intensity of the yellow color is directly proportional to the concentration of OPG in the sample.

A dose-response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated using the values obtained from the standard. The presence of osteoprotegerin in the samples is determined directly from this curve.