The Myostatin ELISA is an enzyme immunoassay intended for the quantitative determination of myostatin in human serum and plasma.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

Developed by Immundiagnostik AG.

Test Principle of the Myostatin ELISA

The Myostatin ELISA is based on the method of a competitive ELISA. A biotinylated myostatin tracer is added to the samples, standards, and controls as a first preparation step. Afterward, aliquots of the treated preparations are transferred and incubated in microtiter plate wells coated with polyclonal anti-myostatin antibodies.

During the first incubation, the free target antigen in the samples competes with the biotinylated myostatin tracer to bind the polyclonal anti-myostatin antibodies immobilized on the microtiter plate wells. The unbound components are removed by a washing step.

During a second incubation step, a streptavidin-labeled-peroxidase antibody, which binds to the biotinylated myostatin tracer, is added to each microtiter well. After a washing step to remove the unbound components, the peroxidase substrate tetramethylbenzidine is added.

Finally, the enzymatic reaction is terminated by an acidic stop solution. The color changes from blue to yellow. The intensity of the yellow color is inversely proportional to the myostatin concentration in the sample. This means a high myostatin concentration in the sample reduces the concentration of the biotinylated myostatin tracer bound to the immobilized anti-myostatin antibodies and lowers the photometric signal.

A dose-response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated using the values obtained from the standard. The presence of myostatin in the samples is determined directly from this curve.