Lactoferrin ELISA

The Lactoferrin ELISA is an enzyme immunoassay intended for the quantitative determination of lactoferrin in stool.

For research use only. Not for use in diagnostic procedures.

For laboratory professional use only.

Manufactured by Immundiagnostik AG.

This kit can be used with the IDK Extract® Stool Collection Tubes: K6999.US

Test Principle of the Lactoferrin ELISA

The Lactoferrin ELISA is intended for the quantitative determination of lactoferrin in stool.

In a first incubation step, the lactoferrin in the samples is bound to polyclonal antibodies, immobilized to the surface of the microtiter wells. To remove all unbound substances, a washing step is carried out.

In a second incubation step, a peroxidase-labeled conjugate (goat anti-human lactoferrin) is added, which specifically recognizes the bound lactoferrin. After another washing step to remove all unbound substances, the solid phase is incubated with the substrate, tetramethyl-benzidine (TMB), which reacts with the peroxidase.

An acidic stop solution is added to stop the reaction. The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of lactoferrin.

A dose-response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated using the values obtained from the standards. The lactoferrin present in the samples is determined directly from this curve.