L-Citrulline Photometric Assay
Available with Lead Time
Sample Type (Matrix): Urine, Serum, Plasma
Sample Volume: 500 µl
Incubation time: 1h 55m
Standard Range: 6.25-400 µmol/l
Size: 96 wells
Regulatory Status: For research use only in the U.S.
Kit Manual: Download
The L-Citrulline Photometric Assay Kit is intended for the quantitative determination of L-citrulline in urine, serum, and plasma.
For research use only. Not for use in diagnostic procedures.
For Laboratory Professional Use Only.
This is the RUO version of Immundiagnostik part number K 6600.
Test Principle of the L-Citrulline Photometric Assay Kit
After pre-treating the sample to eliminate the interference of other substances, a development solution composed of two components is added to the sample. The color changes to intensive red due to the reaction of L-citrulline with DAMO. The interference of reaction byproducts is reduced by TSC-treating. The color intensity is proportional to the analyte concentration. The absorbance is measured at 540 nm. The concentration of the samples is estimated using a standard curve.
An individual sample blank should be run to eliminate the sample matrix's effect on absorption. The obtained blank value is subtracted from the sample result.
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