The IDKmonitor® Vedolizumab Drug Level ELISA is intended for the quantitative determination of free vedolizumab (therapeutic antibody against α4β7 integrin, e.g. ENTYVIO®) in EDTA plasma and serum.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

This is the RUO version of Immundiagnostik part number K 9658.

Browse our other IDKmonitor® Vedolizumab Kits.

Test Principle of the IDKmonitor® Vedolizumab Drug Level ELISA

In the first incubation step, the free vedolizumab from the sample is bound to the specific monoclonal anti-vedolizumab antibody coated on the plate. To remove all unbound substances, a washing step is carried out. In a further incubation step, a Peroxidase-labeled antibody is added. Tetramethylbenzidine (TMB) is used as a substrate for peroxidase. Finally, an acidic stop solution is added to terminate the reaction.

The color changes from blue to yellow. The intensity of the yellow colour is directly proportional to the concentration of free vedolizumab in the sample.

A dose-response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated using the values obtained from the standard. The presence of free vedolizumab in the samples is determined directly from this curve.