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IDKmonitor® Ustekinumab Free ADA ELISA

Item #: KR9666 Categories: ,


Available with Lead Time!

Method: ELISA
Sample Type (Matrix): Serum, EDTA Plasma
Sample Volume: 25 µl
Species: Human
Size: 96 wells
Regulatory Status: For research use only in the U.S.
Kit Manual: Download

The IDKmonitor® Ustekinumab Free ADA ELISA is intended for the determination of free anti-drug antibodies (ADA) against the therapeutic TNF-alpha antibody ustekinumab (e.g. Stelara®) in EDTA plasma and serum.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

This is the RUO version of Immundiagnostik part number K 9666.

We also offer the IDKmonitor® Ustekinumab Drug Level ELISA.

Test Principle of the IDKmonitor® Ustekinumab Free ADA ELISA

In the first incubation step, the free anti-therapeutic antibodies from the sample are bound to the ustekinumab F(ab)2 fragments coated on the plate. To remove all unbound substances, a washing step is carried out.

In a further incubation step, peroxidase-labeled ustekinumab is added. After another washing step to remove all unbound substances, the solid phase is incubated with the substrate, tetramethylbenzidine (TMB). An acidic stop solution is then added.

The color converts to yellow. The absorbance of the color compound is determined photometrically. The intensity of the color is directly proportional to the amount of bound ADAs (here: anti-ustekinumab antibodies) from the sample.

The results are evaluated by a cut-off control.

Additional information

Weight 35.5 oz
Dimensions 6.5 × 10 × 4.5 in
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