IDKmonitor® Infliximab Free ADA ELISA

$895.00

Available on backorder

Product Specifications

Method: ELISA
Sample Type (Matrix): Serum, EDTA Plasma
SAMPLE VOLUME:
50 µL
Species: Human
Incubation time: Overnight
Size: 96 wells
Regulatory Status: For Research Use Only. Not for use in diagnostic procedures.

Description

The IDKmonitor® Infliximab Free ADA ELISA is intended for the qualitative determination of free human antibodies against Infliximab (e.g. REMICADE®) in EDTA plasma and serum.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

If you’re in Canada, we offer a Health Canada Licensed version of this kit: K9650

Manufactured by Immundiagnostik AG.

Also available:

  • Infliximab Total ADA ELISA: KR6954
  • Infliximab Total ADA ELISA (Health Canada): K9654
  • Infliximab Drug Level ELISA: KR9655
  • Infliximab Drug Level ELISA (Health Canada): K9655

Test Principle of the IDKmonitor® Infliximab Free ADA ELISA

This ELISA is designed for the determination of free antibodies against Infliximab (e. g. REMICADE®).

In the first incubation step, the free anti-infliximab antibodies from the sample are bound to the infliximab F(ab)2 fragments coated on the plate. A washing step is carried out to remove all unbound substances. In a further incubation step, a peroxidase-labeled therapy antibody is added. After another washing step to remove all unbound substances, the solid phase is incubated with a tetramethylbenzidine (TMB) substrate. An acidic stop solution is then added.

The color converts to yellow. The absorbance of the color compound is determined photometrically at 450 nm. The intensity of the color is directly proportional to the amount of bound anti-infliximab antibodies (e.g., REMICADE®) from the sample. The results are evaluated by a cut-off control.

 

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