$975.00
Available on backorder
The IDKmonitor® Golimumab Drug Level ELISA is intended for the quantitative determination of free golimumab (therapeutic antibody against TNF-alpha, e.g. SIMPONI®) in EDTA plasma and serum.
For research use only. Not for use in diagnostic procedures.
For Laboratory Professional Use Only.
This is the RUO version of Immundiagnostik part number K 9656.
The IDKmonitor® Golimumab Drug Level ELISA is designed to determine the quantity of free golimumab (therapeutic antibody against TNFα) in EDTA plasma or serum samples.
In the first incubation step, free golimumab from the sample is bound to the specific monoclonal anti-golimumab antibody coated on the plate. To remove all unbound substances, a washing step is carried out. In a further incubation step, a peroxidase-labeled antibody is added. Tetramethylbenzidine (TMB) is used as a substrate for peroxidase. Finally, an acidic stop solution is added to terminate the reaction.
The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of free golimumab in the sample. A dose-response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated using the values obtained from the standard. The concentrations of free golimumab in the samples are determined directly from this curve.
