The IDKmonitor® Golimumab Drug Level ELISA is intended to quantitatively determine free golimumab (therapeutic antibody against TNFα, e.g., SIMPONI®) in EDTA plasma and serum.
Health Canada Licensed.
For Laboratory Professional Use Only.
Not for sale in the U.S. Please contact us to order or get a quote.
If you’re in the U.S., we offer an RUO version of this kit: KR9656
Manufactured by Immundiagnostik AG.
Also available:
The IDKmonitor® Golimumab Drug Level ELISA is designed to determine the quantity of free golimumab (therapeutic antibody against TNFα) in EDTA plasma or serum samples.
In the first incubation step, free golimumab from the sample is bound to the specific monoclonal anti-golimumab antibody coated on the plate. A washing step is carried out to remove all unbound substances. In a further incubation step, a peroxidase-labeled antibody is added. Tetramethylbenzidine (TMB) is used as a substrate for peroxidase. Finally, an acidic stop solution is added to terminate the reaction.
The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of free golimumab in the sample. Using the values obtained from the standard, a dose-response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated. The concentrations of free golimumab in the samples are determined directly from this curve.
