IDKmonitor® Etanercept Drug Level ELISA

The IDKmonitor® Etanercept Drug Level ELISA is an enzyme immunoassay intended for the quantitative determination of free therapeutic TNF-alpha antibodies Etanercept (e.g., ENBREL®) in EDTA plasma and serum.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

Manufactured by Immundiagnostik AG.

Also available:

• Etanercept Free ADA ELISA: KR9653

Test Principle of the IDKmonitor® Etanercept Drug Level ELISA

The IDKmonitor® Etanercept Drug Level ELISA is designed to determine the quantity of free Etanercept (therapeutic antibody against TNFα, e.g., ENBREL®) in EDTA plasma or serum samples.

In the first incubation step, the free Etanercept from the sample is bound to the specific monoclonal anti-Etanercept antibody coated on the plate. A washing step is carried out to remove all unbound substances.

In a further incubation step, a peroxidase-labeled antibody is added. Tetramethylbenzidine (TMB) is used as a substrate for peroxidase.

Finally, an acidic stop solution is added to terminate the reaction. The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of free Etanercept in the sample.

Using the values obtained from the standard, a dose-response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated. Free Etanercept, present in the samples, is determined directly from this curve.