$975.00
Available on backorder
The IDKmonitor® Adalimumab Drug Level ELISA is intended for the quantitative determination of free adalimumab (therapeutic antibody against TNFα, e.g. HUMIRA®) in EDTA plasma and serum.
For research use only. Not for use in diagnostic procedures.
For Laboratory Professional Use Only.
If you’re in Canada, we offer a Health Canada Licensed version of this kit: K9657
Manufactured by Immundiagnostik AG.
Also available:
The IDKmonitor® Adalimumab Drug Level ELISA is designed to quantitate free adalimumab (therapeutic antibody against TNFα, e. g. HUMIRA®) in EDTA plasma or serum samples.
In the first incubation step, the free adalimumab from the sample is bound to the specific monoclonal anti-adalimumab antibody coated on the plate. A washing step is carried out to remove all unbound substances. In a further incubation step, a Peroxidase-labeled antibody is added. Tetramethylbenzidine (TMB) is used as a substrate for peroxidase. Finally, an acidic stop solution is added to terminate the reaction.
The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of free adalimumab in the sample. Using the values obtained from the standard, a dose-response curve of the absorbance unit (optical density, OD) vs. concentration is generated. The concentrations of free adalimumab in the samples are determined directly from this curve.