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The IDK® Zonulin ELISA Bulk Pack (Serum, 20 Plates) is intended for the quantitative determination of zonulin in serum.
For research use only. Not for use in diagnostic procedures.
For Laboratory Professional Use Only.
Please contact us to order or get a quote.
Manufactured by Immundiagnostik AG.
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The IDK® Zonulin ELISA (Serum) is based on the method of competitive ELISA.
As a first preparation step, a biotinylated zonulin tracer is added to the samples, standards, and controls. Afterward, aliquots of the treated samples, standards, and controls are transferred and incubated in microtiter plate wells coated with polyclonal anti-zonulin antibodies. During the incubation, the free target antigen in the samples competes with the biotinylated zonulin tracer for the binding of the polyclonal anti-zonulin antibodies immobilized on the microtiter plate wells. A washing step removes the unbound components.
During a second incubation step, peroxidase-labeled streptavidin, which binds to the biotinylated zonulin tracer, is added to each microtiter well. After a washing step to remove the unbound components, the peroxidase substrate tetramethylbenzidine (TMB) is added.
Finally, the enzymatic reaction is terminated by an acidic stop solution. The color changes from blue to yellow, and the absorbance is measured in the photometer at 450 nm. The intensity of the yellow color is inversely proportional to the zonulin concentration in the sample; this means a high zonulin concentration in the sample reduces the concentration of the biotinylated zonulin tracer bound to the immobilized anti-zonulin antibodies and lowers the photometric signal.
A dose-response curve of absorbance unit (optical density, OD at 450 nm) vs. concentration is generated using the values obtained from the standard. Zonulin, present in the samples, is determined directly from this curve.
