IDK® Pancreatic Elastase ELISA

$600.00

Product Specifications

Method: ELISA
Sample Type (Matrix): Stool
SAMPLE VOLUME:
15 mg
Species: Human
Incubation time: 1h 10m
Standard Range: 2-72 ng/ml
Size: 96 wells
Regulatory Status: FDA Class 1 Exempt. For In Vitro Diagnostic Use. Health Canada Licensed.

Description

The IDK® Pancreatic Elastase ELISA is an enzyme immunoassay intended for the quantitative determination of human pancreatic elastase in stool.

FDA Class 1 Exempt. For In Vitro Diagnostic Use. Health Canada Licensed. For Laboratory Professional Use Only.

This ELISA is compatible with our IDK Extract® Stool Sample Preparation System.

The IDK® Pancreatic Elastase ELISA is also available in a customized bulk pack (20 plates) if your lab is testing at high volumes.

This ELISA was developed by Immundiagnostik AG.

IDK® Pancreatic Elastase ELISA Resources

Download our brochure to discover more.

Test Principle of the IDK® Pancreatic Elastase ELISA

The IDK® Pancreatic Elastase ELISA is intended for the quantitative determination of pancreatic elastase in stool.

In the first incubation step, the pancreatic elastase in the samples is bound to monoclonal antibodies and immobilized to the surface of the microtiter wells. To remove all unbound substances, a washing step is carried out.

In a second incubation step, a peroxidase-labeled conjugate (mouse anti-pancreatic elastase) is added, which specifically recognizes the bound pancreatic elastase. After another washing step to remove all unbound substances, the solid phase is incubated with the substrate, tetramethyl-benzidine (TMB), which reacts with the peroxidase.

Finally, an acidic stop solution is added to stop the reaction. The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of pancreatic elastase.

A dose-response curve of absorbance unit (optical density, OD at 450 nm) vs. concentration is generated using the values obtained from the standards. Pancreatic elastase, present in the samples, is determined directly from this curve.

 

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