The IDK® Pancreatic Elastase ELISA is an enzyme immunoassay intended for the quantitative determination of human pancreatic elastase in stool.

For Laboratory Professional Use Only.

Pancreatic elastase is a proteolytic enzyme produced in the pancreatic acinar cells and then secreted into the duodenum, where it is converted into an active enzyme. Upon activation, pancreatic elastase degrades proteins and plays a vital role in digestion.

This ELISA is compatible with our IDK Extract® Stool Sample Preparation System.

Features of the IDK® Pancreatic Elastase ELISA

• Pancreatic elastase is not broken down during passage through the GI tract, making fecal pancreatic elastase an ideal marker for EPI.
• Fecal pancreatic elastase is 5-6 fold more concentrated than in pancreatic juice.
• FDA Class 1 Exempt. For In Vitro Diagnostic Use. Health Canada Licensed.

The IDK® Pancreatic Elastase ELISA is also available in a customized bulk pack (20 plates) if your lab is testing at high volumes.

This ELISA was developed by Immundiagnostik AG.

Test Principle of the IDK® Pancreatic Elastase ELISA

The IDK® Pancreatic Elastase ELISA is intended for the quantitative determination of pancreatic elastase in stool.

In the first incubation step, the pancreatic elastase in the samples is bound to monoclonal antibodies and immobilized to the surface of the microtiter wells. To remove all unbound substances, a washing step is carried out.

In a second incubation step, a peroxidase-labeled conjugate (mouse anti-pancreatic elastase) is added, which specifically recognizes the bound pancreatic elastase. After another washing step to remove all unbound substances, the solid phase is incubated with the substrate, tetramethyl-benzidine (TMB), which reacts with the peroxidase.

Finally, an acidic stop solution is added to stop the reaction. The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of pancreatic elastase.

A dose-response curve of absorbance unit (optical density, OD at 450 nm) vs. concentration is generated using the values obtained from the standards. Pancreatic elastase, present in the samples, is determined directly from this curve.