IDK® Hemoglobin ELISA

The IDK® Hemoglobin ELISA is intended for the quantitative determination of human hemoglobin in stool.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

Manufactured by Immundiagnostik AG.

This kit can be used with the IDK Extract® Stool Collection Tubes: K6999.US

Also available:

• Hemoglobin ELISA Bulk Pack (20 Plates): KR7816D.20
• Hemoglobin/Haptoglobin Complex ELISA: KR7817D
• Hemoglobin/Haptoglobin Complex ELISA (1-point-calibration): KR7837D

Test Principle of the IDK® Hemoglobin ELISA

This test is used for the quantitative determination of hemoglobin in stool.

The hemoglobin in the sample is bound to anti-hemoglobin antibodies (in excess), which are immobilized on the surface of the microtiter wells. A washing step is carried out to remove all unbound substances. In a second incubation step, an anti-hemoglobin peroxidase-labeled antibody is added. After another washing step to remove all unbound substances, the solid phase is incubated with the substrate, tetramethylbenzidine.

An acidic solution is then added to stop the reaction. The color converts from blue to yellow. The intensity of the yellow color is directly proportional to the hemoglobin concentration in the sample. A dose-response curve of the absorbance unit (optical density, OD) vs. concentration is generated using the values obtained from the standard. The presence of hemoglobin in the samples is determined directly from this curve.