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IDK® H. Pylori Antigen ELISA (Health Canada)

Item #: K 6923 Category:


Available with Lead Time!

Method: ELISA
Sample Type (Matrix): Stool
Sample Volume: 100 mg or 200 mg
Species: Human
Incubation time: 1h 10m
Size: 96 wells
Regulatory Status: Health Canada Licensed. Not for sale in the U.S.
Kit Manual: Download

The IDK® H. Pylori Antigen ELISA is a sandwich ELISA for the determination of helicobacter pylori in stool.

The IDK® H. Pylori Antigen ELISA is Health Canada Licensed. We also offer a version for Research Use Only.

For Laboratory Professional Use Only.

H. pylori is regarded as a causative factor for chronic B-gastritis, drug-unrelated ulcus duodeni, and as an etiologic stimulus of gastric MALT-lymphoma. Furthermore, it is suspected of being involved in the pathogenesis of stomach carcinoma. The epidemiology of infection by Helicobacter pylori has been characterized in western industrial nations by a linear increase with age. In developing countries, a large number of children and juveniles are affected. The currently used methods for the diagnosis of Helicobacter pylori infection are very sensitive and highly specific, but all include either invasive sampling or require special technical devices.

Immundiagnostik AG developed the IDK® H. Pylori Antigen ELISA for the detection of H. pylori infection from fecal samples. This cost-efficient and non-invasive methodology provides a reliable result without the loss of sensitivity or specificity.

Test Principle of the IDK® H. Pylori Antigen ELISA

A microtiter plate is coated with antibodies specific to Helicobacter pylori. The Helicobacter pylori antigen in the sample is bound by the immobilized antibodies during the first incubation step. At the same time, a peroxidase-labeled antibody binds the Helicobacter pylori antigen. After a washing step, tetramethylbenzidine is used as substrate for peroxidase.

Finally, an acidic stop solution is added to terminate the reaction. The color changes from blue to yellow. The intensity of the color is proportional to the amount of analyte (sample or control). The results are evaluated by comparison with a cut-off value.

Additional information

Weight35.5 oz
Dimensions6.5 × 10 × 4.5 in
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