The IDK® H. Pylori Antigen ELISA is a sandwich ELISA for the determination of helicobacter pylori in stool.
Health Canada Licensed.
For Laboratory Professional Use Only.
Not for sale in the U.S. Please contact us to order or get a quote.
If you’re in the U.S., we offer an RUO version of this kit: KR6923
Manufactured by Immundiagnostik AG.
A microtiter plate is coated with antibodies specific to Helicobacter pylori. The Helicobacter pylori antigen in the sample is bound by the immobilized antibodies during the first incubation step. At the same time, a peroxidase-labeled antibody binds the Helicobacter pylori antigen. After a washing step, tetramethylbenzidine is used as substrate for peroxidase.
Finally, an acidic stop solution is added to terminate the reaction. The color changes from blue to yellow. The intensity of the color is proportional to the amount of analyte (sample or control). The results are evaluated by comparison with a cut-off value.