The IDK® H. Pylori Antigen ELISA is a sandwich ELISA for the determination of helicobacter pylori in stool.
The IDK® H. Pylori Antigen ELISA is Health Canada Licensed. For Laboratory Professional Use Only.
Not for sale in the U.S. Please contact us to order or get a quote.
If you are in the U.S., you can purchase the RUO version of the H. pylori Antigen ELISA.
Immundiagnostik AG developed the IDK® H. Pylori Antigen ELISA for the detection of H. pylori infection from fecal samples. This cost-efficient and non-invasive methodology provides a reliable result without the loss of sensitivity or specificity.
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A microtiter plate is coated with antibodies specific to Helicobacter pylori. The Helicobacter pylori antigen in the sample is bound by the immobilized antibodies during the first incubation step. At the same time, a peroxidase-labeled antibody binds the Helicobacter pylori antigen. After a washing step, tetramethylbenzidine is used as substrate for peroxidase.
Finally, an acidic stop solution is added to terminate the reaction. The color changes from blue to yellow. The intensity of the color is proportional to the amount of analyte (sample or control). The results are evaluated by comparison with a cut-off value.
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