IDK® Bile Acids Photometric ELISA (Microtiter Plate) (Serum)

The IDK® Bile Acids Photometric ELISA (Microtiter Plate) (Serum) is intended for the quantitative determination of bile acids in serum.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

Developed by Immundiagnostik AG.

Also available:

• Bile Acids Photometric Kit (Microtiter Plate, Stool): KR7878W
• Bile Acids Photometric Assay Kit (Cuvette, Stool): KR7878CV
• Bile Acids Photometric ELISA (Cuvette, Serum): KR7877CV

Test Principle of the IDK® Bile Acids Photometric ELISA

In the presence of excess thio-NAD, bile acids are converted to 3-keto steroids by the enzyme 3-a-hydroxysteroid dehydrogenase while thio-NADH is formed. The rate of formation of thio-NADH can be determined by the change of absorbance (DOD) at 405 nm.

A dose-response curve DOD vs. concentration is generated, using the values obtained from measured standards. The bile acids concentration of the samples is determined directly from this curve.