IDK® anti-heTG IgA ELISA

The IDK® anti-heTG IgA ELISA is an enzyme immunoassay intended for the quantitative determination of IgA anti-epidermal-transglutaminase antibodies in serum and plasma.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

This is the RUO version of Immundiagnostik part number K 9396.

Test Principle of the IDK® anti-heTG IgA ELISA

The IDK® anti-heTG IgA ELISA is designed for the quantitative determination of anti-epidermal transglutaminase antibodies (IgA anti-heTG).

The wells of the microtiter plate are coated with the antigen. In the first incubation step, the anti-heTG antibodies are bound to the coated antigen. To remove all unbound substances, a washing step is carried out. In a further incubation step, a peroxidase-labeled antibody is added.

After another washing step, to remove all unbound substances, the solid phase is incubated with the peroxidase substrate, tetramethylbenzidine (TMB). Finally, an acidic stop solution is added to terminate the enzymatic reaction, whereby the color changes from blue to yellow.

The intensity of the yellow color is directly proportional to the IgA anti-epidermal-transglutaminase antibody concentration. The results are evaluated by a cut-off control.