The IDK® alpha-1-Antitrypsin Clearance ELISA is an enzyme immunoassay intended for the quantitative determination of alpha-1-Antitrypsin in serum, plasma, and stool.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

This is the RUO version of Immundiagnostik part number K 6752.

Test Principle of the IDK® alpha-1-Antitrypsin Clearance ELISA

The IDK® alpha-1-Antitrypsin Clearance ELISA is designed for the quantitative determination of α1-Antitrypsin in serum, plasma, and stool. The assay utilizes the sandwich technique with two selected antibodies that bind to human α1-antitrypsin. Standards, controls, and prediluted samples assayed for human α1-antitrypsin are added into the wells of a microplate coated with a high affine anti-human α1-antitrypsin antibody.

During the first incubation step, α1-antitrypsin is bound by the immobilized antibody. Then a peroxidase-conjugated polyclonal anti-human α1-antitrypsin antibody is added into each microtiter well, and a sandwich of capture antibody – human α1-antitrypsin – peroxidase-conjugate is formed. Tetramethylbenzidine is used as a peroxidase substrate. Finally, an acidic stop solution is added to terminate the reaction. The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of α1-antitrypsin.

A dose-response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated using the values obtained from the standard. α1-antitrypsin present in the samples is determined directly from this curve.