The Human Vitamin B12 CLIA Kit is a Chemiluminescence Immunoassay (CLIA) intended for the quantitative measurement of human Vitamin B12 in serum.
Compatible with the ECL100 Chemiluminescent Immunoassay Analyzer.
For research use only. Not for use in diagnostic procedures.
For lab professional use only.
Manufactured by Epitope Diagnostics, Inc.
Please contact us for a quote.
The Human Vitamin B12 CLIA Kit is designed, developed, and produced for the quantitative measurement of human VB 12 level in serum samples. The assay utilizes competition law for testing.
The calibrators, controls, or sample, monoclonal antibody to B12 labeled with microparticles are mixed and incubated, forming an antigen-antibody immuno-complex. Acridinium ester labeled B12 are added and incubated. Acridinium ester labeled B12 unbound to the sample binds to the Magnetically microgranular B12 antibody. After precipitation in a magnetic field, the supernatant is decanted, and then a wash cycle is performed to remove any remaining substances that are not bound to the magnetic microbeads.
Subsequently, the washed compound is sent into the measurement chamber where trigger solution is automatically added to initiate a chemiluminescence reaction. The light signal is measured by a photomultiplier as relative light units (RLUs), which is inversely proportional to the concentration of B12 present in the sample.
The test result is automatically calculated by the system according to the working curve.
